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For more information on the Neuropixels probes themselves, check out this wiki set up by Nick Steinmetz of UCL.
Compatibility
The Neuropixels module currently only works with Phase IIIA probes (the pre-production version made by IMEC through mid-2018). These probes require a basestation connected to a KC705 evaluation board in order to communicate with a computer (via Ethernet). A version that is compatible with Phase IIIB probes (and associated PXIe hardware) is under development.
Overview
This page contains a brief guide to configuring the Open Ephys GUI for use with IMEC Neuropixels Phase IIIA probes.
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The Windows executable can be downloaded from https://github.com/open-ephys-GUI-binaries/open-ephys/tree/neuropix.
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| NOTE: A newer version of the GUI with features such as spike raster and PSTH display is available at https://github.com/open-ephys-GUI-binaries/open-ephys/tree/neuropix-2. However, this version has not been thoroughly tested, and is not yet recommended for experiments. |
It’s possible to download all the necessary files as a ZIP archive, using the “Download ZIP” button in the upper right. But we strongly recommend using the GitHub Desktop Client, so it’s easier to ensure you’re running the latest version.
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For Option III & IV probes, you can select which channels to acquire data from by clicking on those channels and pressing the ENABLE button. For every channel that is enabled (yellow), another channel is disabled (red). There will eventually be an option to disable the output of individual channels (using the ON/OFF buttons) so their data doesn’t flow through the signal chain, but this has not been implemented yet.
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| NOTE: The "Probe Option" info label may be off by 1 in some versions of the GUI, e.g., Option III probes will appear as Option II probes. |
Use the four drop-down menus to set the AP gain, LFP gain, reference, and filter cutoff. Currently, these parameters are set globally. Changing any of these parameters takes approximately 1 second.
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