...
The Windows executable can be downloaded from https://github.com/open-ephys-GUI-binaries/windowsopen-32ephys/tree/neuropix.
It’s possible to download all the necessary files as a ZIP archive, using the “Download ZIP” button in the upper right. But we strongly recommend using the GitHub Desktop Client, so it’s easier to ensure you’re running the latest version.
...
Inside the directory (called “windows“open-32,” ephys” or “windows“open-32ephys-neuropix” if you downloaded the ZIP file), you can run the application by launching open-ephys.exe. The executable must remain in this directory in order to access the associated DLL files.
...
To load the probe’s gain calibration settings from EEPROM, press the CALIBRATE button ADC CALIBRATION and GAIN CALIBRATION buttons. This must be done before every recordingevery time the probe is powered on, and can take up to 10 minutes. The GUI will be frozen during this time, so please be patient! You’ll know it’s finished when the button icon changes color.
...
Open the display by clicking the tab or window button at the top of the processor. All the data streaming from the Neuropix module will now be visible in the LFP Viewer. You can change the timebase, input range, channel spacing, or color scheme of the display. Extra options are exposed by clicking the arrow button. We recommend using an input range of 250 uV and a channel spacing (size) of 50 pixels. The "DC OFFSET" button will automatically subtract the constant voltage offset from each channel (for display purposes only).
5. Acquiring and recording data
...
The default format is the "Binary" format, which writes the data to a flat binary file of int16s in the form "ch1 sample 1, ch2 sample 1, ... ch384 sample 1, ch1 sample2, etc." There are separate files for the AP band (30 kHz) and LFP band (2.5 kHz). We recommend using this format because it's efficient and directly compatible with Kilosort. Events are saved in the 'all_channels.events" file. More info on the format can be found here. To convert from the stored int16 values to microvolts, simply multiply by 0.195.
...