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Phase 3a

Neuropixels 1.0

Manufactured between2016-2018

2018-present






Sold toNeuropixels consortiumGeneral public
Site layoutcheckerboardcheckerboard or linear
Shank length5 mm (options 1 & 2); 10 mm (options 3 & 4)10 mm
Site count384-960960
References1 external, 10-30 internal1 external, 1 tip, 3 internal
CableDual coax (white)Single twisted pair (black and yellow)
Acquisition systemEthernet (based on KC705 eval board)PXIe
Max probes per computer316
Compatible softwareSpikeGLX, Open Ephys GUISpikeGLX, Open Ephys GUI
Open Ephys pluginNeuropix-3aNeuropix-PXI

Combining Neuropixels with the Open Ephys GUI

Both the Neuropixels 3a and PXI plugins are included by default in the Windows version of the GUI (download .zip file).

Once you've unzipped the directory, you can run the application by launching open-ephys.exe. The executable must remain in this directory in order to access the associated DLL files, but you can create a link that resides elsewhere.

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Note: If the GUI won't start because of missing DLLs, you'll need to install the Visual Studio Redistributable Package.

Once you launch Open Ephys, you can connect to your probe(s) by dragging and dropping the appropriate plugin onto the signal chain. If you're using the "Neuropixels 1.0" probes with a PXIe data acquisition system, use the Neuropix-PXI plugin. If you're using "Neuropixels 3a" probes, use theĀ Neuropix-3a plugin.

For more information on how to use the Open Ephys GUI, please check out this tutorial.