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Cut 16 (or fewer) segments of 33ga polyimide tubes to around 6 cm length. This size of polyimide tube results in average electrode pitches of ~250 micron, though electrodes are free to move within its guide tube.

 

Glue the guide tubes into rows with superglue and arrange rows to form linear arrays


Make

Align a row of the polyimide tubes across a strip of double-sided tape making sure there are no gaps between adjacent tubes. Using a section of the same tubing, apply a very small amount of thin superglue at the intersection of two adjoining tubes and then gently press down the next layers’ tube on top with fine forceps. The glue will quickly spread up and down the intersection of the two tubes, make sure that no glue gets
into the

tubes,

bottom of the tube and that the tubes are free from glue above a consistent depth so they are free to fan out at that depth (if glue spreads up too far you can manually fan the tubes out before it fully dries).

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For guide tubes that are not supported by a layer of tubes from below add shorter lengths of tubing to prop them up.

If you’re having trouble attaching a tube, it can be helpful to also glue new tubes to the tubes adjacent to them (assuming you have already partially completed the layer).

If the second layer of your array will be wider than the bottom layer, place additional, shorter sections of tubes on the sides bottom layer so you have something to glue the second layer onto.

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To add a fiber to the array later, coat a piece of fiber or a steel cannula of the same diameter with mineral oil, and arrange into array like a guide tube. This will function as a place holder. Make sure that the type and amount of oil works for ensuring that the cannula can later be removed - test this first to avoid destroying a guide tube array.

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