Explain basic steps for successful durotomy in mice
needed because of: regrowth, serial lowering into brain, avoid bunching of trodes
Protocol:
Drill a craniotomy, before lifting the bone, cover area in (cooled to suppress swelling) saline/acsf to avoid any air getting in contact with the dura, even for very short periods - any dried areas will stick to the brain, making a clean removal impossible.
Use cooled saline while drilling to avoid heating up the tissue and to control swelling.
Carefully lift the bone while under saline/acsf.
Stop any bleeding with gelfoam and/or carefully flush out blood. Also keep flushing until there is no bone dust or other debris on the surface of the liquid.
(The next steps are assuming a left craniotomy on SI, and a right-handed surgeon, modify as required.)
Pick a spot with no surface vessels on the right edge of the craniotomy and make a very small incision into the dura with a fresh needle.
Carefully insert the lower jaw of a ceramic coated dumont #5 forceps (available at FST) into the incision, almost tangential to the brain. The goal is to insert the tool under the dura as far as possible and to carefully separate it from the brain by moving the forceps laterally.
Once enough dura has been detached from the brain (ideally you should have loosened up the medial half of the craniotomy by now, all the way to the rostral and caudal ends of the craniotomy) carefully lift the tool a bit to suction more saline under the still attached flap of dura. This can help in detaching more dura.
Occasionally, the dura seems lightly attached to the underlying tissue at the sides of large surface vessels, and lifting it off can cause bleeding. In the few cases that we encountered this, the bleeding could be stopped relatively easily with gelfoam, but extra caution is needed when this happens to avoid damaging the big blood vessels when lifting the dura.
Pull off dura at one side of the craniotomy and pull the resulting flap off to the opposite side. Avoid creating small free floating segments of dura that could settle in the area of electrode placement!
Repeat the process with the remaining, more lateral part of the dura.
When finished, rinse with more cooled saline/acsf and keep covered until ready to implant drive. It might be simplest to cover the craniotomy with surgilube or a oil/paraffin medium to keep the brain protected until the implant.