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Combining Neuropixels with the Open Ephys GUI
The Neuropixels-specific Both the Neuropixels 3a and PXI plugins are included by default in the Windows version of the Open Ephys GUI can be downloaded from https://github.com/open-ephys-GUI-binaries/open-ephys/tree/neuropix.
You can download all the necessary files as a ZIP archive, using the “Download ZIP” button in the upper right. Alternatively, you can use the GitHub Desktop Client found at https://desktop.github.com to save a copy of the repository to your machine. Make sure the “neuropix” branch is active before you do so. Any future software updates can be downloaded by pressing the “Sync” button inside the Desktop Client.
Either way, we recommend creating a separate copy of the GUI for running experiments, which will give you a chance to test out any new features before using them to collect real data.
Inside the directory (called “open-ephys” or “open-ephys-neuropix” if you downloaded the ZIP file)GUI (download .zip file).
Once you've unzipped the directory, you can run the application by launching open-ephys.exe. The executable must remain in this directory in order to access the associated DLL files, but you can create a link that resides elsewhere.
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Note: If the GUI won't start because of missing DLLs, you'll need to install the Visual Studio 2013 Visual C++ Redistributable Package.
Once you launch Open Ephys, you can connect to your probe(s) by dragging and dropping the appropriate plugin onto the signal chain. If you're using the "Neuropixels 1.0" probes with a PXIe data acquisition system, use the Neuropix-PXI plugin. If you're using "Neuropixels 3a" probes, use the Neuropix-3a plugin.
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