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Overview

Neuropixels are high-density silicon probes manufactured by IMEC. For information on how to order them, please visit neuropixels.org.

Currently, Neuropixels probes come in two varieties, "Phase 3a" and "Neuropixels 1.0." The following table compares these two types of probes.


Phase 3a

Neuropixels 1.0

Manufactured between2016-2018

2018-present






Sold toNeuropixels consortiumGeneral public (via lottery system)
Site layoutcheckerboardcheckerboard or linear
Shank length5 mm (options 1 & 2); 10 mm (options 3 & 4)10 mm
Site count384-960960
References1 external, 10-30 internal1 external, 1 tip, 3 internal
CableDual coax (white)Single twisted pair (black and yellow)
Acquisition systemEthernet (based on KC705 eval board)PXIe
Max probes per computer316
Compatible softwareSpikeGLX, Open Ephys GUISpikeGLX, Open Ephys GUI
Open Ephys pluginNeuropix-3aNeuropix-PXI



Combining Neuropixels with the Open Ephys GUI

The Neuropixels-specific version of the Open Ephys GUI can be downloaded from https://github.com/open-ephys-GUI-binaries/open-ephys/tree/neuropix.

It’s possible to download all the necessary files as a ZIP archive, using the “Download ZIP” button in the upper right. But we strongly recommend using the GitHub Desktop Client, so it’s easier to ensure you’re running the latest version.

To do this, install the GitHub Desktop Client found at https://desktop.github.com, then use the “Clone in Desktop” button to save a copy of the repository to your machine. Make sure the “neuropix” branch is active before you do so.

Any future software updates can be downloaded by pressing the “Sync” button inside the Desktop Client.

Inside the directory (called “open-ephys” or “open-ephys-neuropix” if you downloaded the ZIP file), you can run the application by launching open-ephys.exe. The executable must remain in this directory in order to access the associated DLL files, but you can create a link that resides elsewhere.


Note: If the GUI won't start because of missing DLLs, you'll need to install the Visual Studio 2013 Visual C++ Redistributable Package.

Once you launch Open Ephys, you can connect to your probe(s) by dragging and dropping the appropriate plugin onto the signal chain. If you're using the "Neuropixels 1.0" probes with a PXIe data acquisition system, use the Neuropix-PXI plugin. If you're using "Neuropixels 3a" probes, use the Neuropix-3a plugin.

For more information on how to use the Open Ephys GUI, please check out this tutorial.



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